NITROGEN LIMITATION AFFECTS THE NITROGEN — ASSIMILATORY PATHWAY AT THE TRANSCRIPTOME LEVEL

We identified a number of genes that encode for components of the ni­trogen assimilatory pathway (Table 4). Genes that encode for enzymes catalyzing the reduction of NO3- to NH4+ and the biosynthesis of nitrogen­carrying amino acids were strongly expressed under nitrogen limitation [32,33]. Along with the pentose phosphate pathway, these genes were among the most up-regulated genes in — N cells of N. oleoabundans. The increased expression of these genes was consistent with their role in nitro­gen uptake and assimilation, and the nitrogen limited growth environment from which cells were derived.

TABLE 4: N. oleoabundans genes involved in nitrogen assimilation

Nitrogen assimilation

Log2FC

High affinity nitrate transporters

-4.4

Ammonium transporters

-2.8

Nitrate reductase (NR, EC: 1.7.1.1)

-3.8

Ferredoxin-nitrite reductase (NiR, EC: 1.7.7.1)

-3.9

Glutamine synthetase (GS, EC: 6.3.1.2)

-2.3

Glutamate synthase (NADH) (GOGAT, EC: 1.4.1.13-14)

-1.4

Glutamate synthase (Ferredoxin) (EC: 1.4.7.1)

0.27

Glutamate dehydrogenase (GDH, EC: 1.4.1.3)

0.89

Aspartate aminotransferase (aspat, EC: 2.6.1.1)

-2.3

Asparagine synthetase (AS, EC: 6.3.5.4)

-1.5

Negative Log2FC values represent up-regulation under nitrogen limitation. All presented fold changes are statistically significant, q value < 0.05.

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