Category Archives: Biomass Recalcitrance

HETEROGLUCANS (xyloglucans)

Xyloglucans are components of the primary walls of most seed plants examined so far. Fucogalactoxyloglucans are the commonest structural type and occur in the primary walls of coniferous gymnosperms, eudicotyledons and in the non-commelinid monocotyledons, at least in onion (Allium cepa) and garlic (A. sativa) (3). These xyloglucans have a re­peating backbone subunit consisting of four (1^4)-linked (3-D-Glcp residues, with […]

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Cell wall anatomy

Maize (Zea mays L.) is a monocotyledonous plant whose anatomical structure has been well studied. It resembles other grasses in the arrangement of tissues in the stem, leaf, and Biomass Recalcitrance: Deconstructing the Plant Cell Wall for Bioenergy. Edited by Michael. E. Himmel © 2008 Blackwell Publishing Ltd. ISBN: 978-1-405-16360-6

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Advanced approaches for characterizing cell wall structure

Whereas plant cell walls have been measured by electron microscopy (EM) (13, 32-36), the drawback of EM techniques lies in the sample preparation and imaging processes commonly used, which involve chemical extraction, dehydration, and embedding. Because biomass samples go through both chemical pretreatment and enzymatic hydrolysis, methods must be developed to minimize the disruption of sample preparation, and conduct imaging […]

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