Category Archives: BIOMASS NOW — CULTIVATION AND UTILIZATION

Sitka stream water column CH4 budget for the experimental stretch of a stream

The potentially important source and sinks terms for dissolved methane in the water column of the Sitka stream are shown in Figure 6. Previously calculated rates of inputs (benthic fluxes) and loss of dissolved CH4 through evasion to the atmosphere can be combined together with advection inputs and losses to yield a CH4 dynamics (budget) for any particular section of […]

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Carbon isotopic composition of dissolved methane and carbon dioxide in sediments

Interstitial water samples for carbon isotopic analysis of methane and carbon dioxide were collected in 2010 — 2011 through three courses at study site. Sampling was performed by set of minipiezometers placed in a depth of 20 to 60 cm randomly in a sediment. After sampling, refrigerated samples were transported (within 72 hours) in 250 mL bottles to laboratory at […]

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Fluorescence in situ hybridization (FISH)

Both methanogenic archaea and aerobic methanotrophs were found at all localities along the longitudinal stream profile. The proportion of these groups to the DAPI-stained cells was quite consistent and varied only slightly but a higher proportion to the DAPI-stained cells in deeper sediment layer 25-50 cm was observed. On average 23,4 % of DAPI-stained cells were detected by FISH with […]

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Nutrient availability

Limited nutrient availability influences negatively biomass production. Nitrogen deficiency strongly depresses vegetation flush. According to Boussadia et al. (2010) [8], total biomass of two olive cultivars (‘Meski’ and ‘Koroneiki’) was strongly reduced (mainly caused by a decrease in leaf dry weight) under severe N deprivation, while in an out-door pot-culture experiment with castor bean plants (Ricinus communis L.), conducted by […]

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Abundance of microbial cells and microbial community composition

For measuring of microbial parameters, formaldehyde fixed samples (2 % final conc.) were first mildly sonicated for 30 seconds at the 15 % power (sonotroda MS 73, Sonopuls HD2200, Sonorex, Germany), followed by incubation for 3 hours under mild agitation with 10 mL of detergent mixture (Tween 20 0.5%, vol/vol, tetrasodium pyrophosphate 0.1 M and distilled water) and density centrifugation […]

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Denaturing gradient gel electrophoresis and cloning

Methanogenic communities associated with hyporheic sediments at two different depths (0­25 cm and 25-50 cm) along the longitudinal stream profile were compared based on the DGGE patterns. As shown in Fig. 9, the DGGE patterns varied highly among study localities (Fig. 9A), irrespective of the depth (Fig. 9B). However, presence of the bands in all samples indicates that methanogens may […]

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Genotypic factors (root morphology and architecture, genetic growth capacity e. t. c.)

According to Bayuelo-Jimenez et al. (2011) [3], under P deficiency, P-efficient accessions of maize plants (Zea mays L.) had greater root to shoot ratio, nodal rooting, nodal root laterals, nodal root hair density and length of nodal root main axis, and first-order laterals. In our experiments, we also found differential root system morphology among three Greek olive cultivars (the root […]

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Procaryotic community composition

The methanogenic archaea, three selected methanogen families (Methanobacteriaceae, Methanosetaceae and Methanosarcinaceae) and methanotrophic bacteria belonging to groups I and II were detected using FISH (Fluorescence in situ hybridization) with 16S rRNA — targeted oligonucleotide probe labelled with indocarbocyanine dye Cy3. The prokaryotes were hybridized according to the protocol by Pernthaler et al. (2001). Briefly, the supernatants which were used also […]

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Occurence of methane in stream water and sediments

In spite of commonly held view of streams as well-oxygenated habitats, we found both surface and interstitial water to be supersaturated with methane compared to the atmosphere at all five localities (Mach et al. in review). Availability of interstitial habitats for bacteria and archaea carrying out anaerobic processes has been confirmed by our previous (Hlavacova et al. 2005, 2006; Cupalova […]

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